Nitrotyrosine Antibody, IgG2a, Clone: [39B6], Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: Nitrotyrosine Antibody, IgG2a, Clone: [39B6], Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB1822475
• Supplier Catalog Number: orb1822475
• Alternative Catalog Number: BYT-ORB1822475-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: AM, ELISA, FC, ICC, IF, IHC, IP, WB
• Species Reactivity: All
• Immunogen: 3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin
• Conjugation: Unconjugated
• Alternative Names: Nitrotyrosine, Nitro tyrosine, 3-Nitrotyrosine

Product Description

Mouse Anti- Nitrotyrosine Monoclonal IgG2a

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Clone Designation: [39B6]
• Isotype: IgG2a
• Buffer: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Target: Nitrotyrosine

Application Notes

• Application Dilute: WB (1:1400), IHC (1:100), optimal dilutions for assays should be determined by the user.
• Application Notes: Application Notes: 0.7 µg/ml of SMC-154 was sufficient for detection of 5 µg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody

Images

Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6. Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red).

Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:1000000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x. With anti-microbial.

Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6. Tissue: liver tissue. Species: Rat. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green).

Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.

Western Blot analysis of Human A549 cells showing detection of Multiple Bands Nitrotyrosine protein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6. Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:1000 for 2.5 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.

Western Blot analysis of Human Recombinant Protein showing detection of Multiple Bands Nitrotyrosine protein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6. Lane 1: MW Ladder. Lane 2: hASYN Monomer (3.84 ug). Lane 3: Nitrosylated hASYN (3.84 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 2 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.

Western Blot analysis of Human HEK293 cells showing detection of Nitrotyrosine protein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6. Lane 1: MW Ladder. Lane A: Nitrosylated-HEK293 (15uL). Lane B: HEK293 (15 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody diluted in 1.5% BSA and TBST for 1 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG: HRP at 1:4000 for 1 hour at RT with shaking. Predicted/Observed Size: Multiple Bands.

Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.