Recombinant full-length human protein was used as the immunogen for the Transmembrane protease serine 2 antibody.
Conjugation:
Unconjugated
Extracellular proteases mediate the digestion of neighboring extracellular matrix components in initial tumor growth, allow desquamation of tumor cells into the surrounding environment, provide the basis for invasion of basement membranes in targeted metastatic organs and are required for release and activation of many growth and angiogenic factors. The TMPRSS2 gene encodes a 492 amino acid multimeric serine protease, which is mainly expressed in the mouse prostate and kidney, and is also expressed in the human small intestine, prostate, colon, stomach and salivary gland. TMPRSS2 contains several domains, including a serine protease domain of the S1 family, a scavenger receptor cysteine-rich domain of group A, an LDL receptor class A domain and a transmembrane domain. TMPRSS2 is expressed as a full length form and a cleaved protease domain and its expression is increased by androgenic hormones. TMPRSS2 is also expressed in prostate carcinoma, suggesting that it may play a role in prostate carcinogenesis.
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Application Dilute:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
Application Notes: Optimal dilution of the Transmembrane protease serine 2 antibody should be determined by the researcher
IHC staining of FFPE human prostate tissue with Transmembrane protease serine 2 antibody (clone TMPRSS2/7420). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Transmembrane protease serine 2 antibody (clone TMPRSS2/7420) as confirmation of integrity and purity.
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