CD4 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: CD4 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB182470
• Supplier Catalog Number: orb182470
• Alternative Catalog Number: BYT-ORB182470-100,BYT-ORB182470-200,BYT-ORB182470-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Porcine
• Immunogen: KLH conjugated synthetic peptide derived from human CD4 (385-457/457aa)
• Conjugation: Unconjugated
• Alternative Names: CD4(L3T4), CD4 antigen(p55), CD4 Antigen, CD4 molecule, CD4 Receptor, CD4+Lymphocyte deficiency, included, CD4mut, L3T4, Leu3, Ly-4, Lymphocyte antigen CD4, MGC165891, p55, T Cell Antigen T4, T cell antigen T4/LEU3, T cell differentiation antigen L3T4, T cell OKT4 deficiency, included, T cell surface antigen T4/Leu 3, T cell surface antigen T4/Leu3, T Cell Surface Glycoprotein CD4, W3/25, W3/25 antigen, T-cell surface glycoprotein CD4 isoform 1 precursor, CD4_HUMAN.

Product Description

CD4 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 48 kDa
• UniProt: P01730
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: CD4

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test

Images

Blank control (blue line): HL60 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-CD4 antibody (orb182470), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (orb182470) at 1:4000 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (orb182470) at 1:4000 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody at 1:300 for one hour at room temperature.

Sample: Lane 1: U937 (Human) Cell Lysate at 30 ug, Lane 2: HL-60 (Human) Cell Lysate at 30 ug, Lane 3: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-CD4 (orb182470) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.

Sample: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-CD4 (orb182470) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 55kD.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-CD4 (orb182470) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 55 kD.

Western blot analysis of HL60 Cell Lysate using CD4 antibody.

Western blot analysis of U937 Cell Lysate using CD4 antibody.