Cdc25C Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Cdc25C Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB182929
• Supplier Catalog Number: orb182929
• Alternative Catalog Number: BYT-ORB182929-100,BYT-ORB182929-200,BYT-ORB182929-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Cdc25C (321-420/473aa)
• Conjugation: Unconjugated
• Alternative Names: CDC 25, Cdc 25C, CDC25, Cell division cycle 25 homolog C, Cell division cycle 25C, Cell division cycle 25C protein, Dual specificity phosphatase Cdc25C, M phase inducer phosphatase 3, Mitosis inducer CDC25, MPIP3, phosphotyrosine phosphatase, MPIP3_HUMAN.

Product Description

Cdc25C Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 53 kDa
• UniProt: P30307
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: CDC25C

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500

Images

A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Cdc25C) polyclonal Antibody, Unconjugated (orb182929) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Cdc25C) polyclonal Antibody, Unconjugated (orb182929) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Cdc25C) polyclonal Antibody, Unconjugated (orb182929) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cdc25C) Polyclonal Antibody, Unconjugated (orb182929) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cdc25C) Polyclonal Antibody, Unconjugated (orb182929) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cdc25C) Polyclonal Antibody, Unconjugated (orb182929) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cdc25C) Polyclonal Antibody, Unconjugated (orb182929) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cdc25C) Polyclonal Antibody, Unconjugated (orb182929) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.