DTX3L Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: DTX3L Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB183214
• Supplier Catalog Number: orb183214
• Alternative Catalog Number: BYT-ORB183214-100,BYT-ORB183214-200,BYT-ORB183214-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human DTX3L (651-740/740aa)
• Conjugation: Unconjugated
• Alternative Names: B lymphoma and BAL associated protein, B-lymphoma-and BAL-associated protein, BBAP, Deltex 3 like, DTX 3L, DTX3L, DTX3L_HUMAN, E3 ubiquitin-protein ligase DTX3L, Protein deltex 3 like, Protein deltex 3 like protein, Protein deltex-3-like, Rhysin 2, Rhysin-2, Rhysin2.

Product Description

DTX3L Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 83 kDa
• UniProt: Q8TDB6
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: DTX3L

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Immunohistochemical staining of human kidney tissue using DTX3L antibody.

Western blot analysis of mouse intestinal lysate using DTX3L antibody.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DTX3L) Polyclonal Antibody, Unconjugated (orb183214) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DTX3L) Polyclonal Antibody, Unconjugated (orb183214) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DTX3L) Polyclonal Antibody, Unconjugated (orb183214) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DTX3L) Polyclonal Antibody, Unconjugated (orb183214) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DTX3L) Polyclonal Antibody, Unconjugated (orb183214) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-DTX3L Polyclonal Antibody, Unconjugated (orb183214) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.