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Goat polyclonal antibody to NOS1
Clonality:
Polyclonal
Molecular Weight:
161, 164.6, 125
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Application Notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: This product has been sucessfully used for IHC on Olfactory bulb in mice (PMID: 20140458)WB: Approx 160-170kDa band observed in Human Skeletal Muscle and Mouse Brain lysates (calculated MW of 161kDa according to NP_000611.1). Recommended concentration: 0.3-1 µg/ml.Experiment Notes: Immunofluorescence: This product has been successfully used for IF as reported (PMID: 20140458)
1 µg/mL staining of Mouse Brain lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunostaining of 30 µm thick cryosections of PFA-perfused Human Hypothalamus, antigen retrieval with citrate buffer Ph 6 at 80C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.
Immunohistochemical staining of mouse caudate-putamen using NOS1 antibody
Immunohistochemical staining of Human Cortex using NOS1 antibody
Immunofluorescence analysis of HeLa cells using NOS1 antibody
Flow cytometric analysis of Kelly cells using NOS1 antibody
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