LCHN Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: LCHN Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB184869
• Supplier Catalog Number: orb184869
• Alternative Catalog Number: BYT-ORB184869-100,BYT-ORB184869-200,BYT-ORB184869-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Mouse
• Immunogen: KLH conjugated synthetic peptide derived from human LCHN (301-400/455aa)
• Conjugation: Unconjugated
• Alternative Names: KIAA1147, LCHN, LCHN_HUMAN, Protein LCHN.

Product Description

LCHN Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 51 kDa
• UniProt: A4D1U4
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: DENND11

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LCHN) Polyclonal Antibody, Unconjugated (orb184869) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Large intestine (Mouse) Lysate at 40 ug, Primary: Anti-LCHN (orb184869) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.