LGI2 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: LGI2 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB184908
• Supplier Catalog Number: orb184908
• Alternative Catalog Number: BYT-ORB184908-100,BYT-ORB184908-200,BYT-ORB184908-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Mouse
• Immunogen: KLH conjugated synthetic peptide derived from human LGI2 (201-300/545aa)
• Conjugation: Unconjugated
• Alternative Names: KIAA1916, Leucine rich glioma inactivated protein 2, Leucine rich repeat LGI family member 2, Leucine-rich glioma-inactivated protein 2, Leucine-rich repeat LGI family member 2, LGI1 like protein 2, LGI1-like protein 2, LGI2, LGI2_HUMAN, LGIL2.

Product Description

LGI2 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 60 kDa
• UniProt: Q8N0V4
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: LGI2

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LGI2) Polyclonal Antibody, Unconjugated (orb184908) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-LGI2 (orb184908) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD.