LXR alpha + LXR beta Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: LXR alpha + LXR beta Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB185093
• Supplier Catalog Number: orb185093
• Alternative Catalog Number: BYT-ORB185093-100,BYT-ORB185093-200,BYT-ORB185093-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, WB
• Species Reactivity: Human, Mouse
• Immunogen: KLH conjugated synthetic peptide derived from human LXR alpha (51-150/447aa)
• Conjugation: Unconjugated
• Alternative Names: Liver X receptor alpha, Liver X receptor alpha, Liver X receptor beta, LXR a, LXR, LXR b, LXRa, LXRalpha, LXRb, LXRbeta, NER, NERI, NR1H2, NR1H3, Nuclear orphan receptor LXR alpha, Nuclear orphan receptor LXR beta, Nuclear receptor NER, Nuclear receptor subfamily 1 group H member 2, Nuclear receptor subfamily 1 group H member 3, Oxysterols receptor LXR alpha, Oxysterols receptor LXR beta, RIP15, RLD1, Ubiquitously expressed nuclear receptor, UNR.

Product Description

LXR alpha + LXR beta Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 51 kDa
• UniProt: Q13133
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: NR1H3

Application Notes

• Application Dilute: WB=1:500-2000, Flow-Cyt=2ug/Test

Images

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-LXR alpha + LXR beta antibody (orb185093), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-LXR alpha + LXR beta (orb185093) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.