Melanoma gp100 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Melanoma gp100 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB185162
• Supplier Catalog Number: orb185162
• Alternative Catalog Number: BYT-ORB185162-100,BYT-ORB185162-200,BYT-ORB185162-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human
• Immunogen: KLH conjugated synthetic peptide derived from human Melanoma gp100 (21-120/661aa)
• Conjugation: Unconjugated
• Alternative Names: 95 kDa melanocyte specific secreted glycoprotein, 95 kDa melanocyte-specific secreted glycoprotein, D12S53E, gp100, M-beta, ME20, ME20 M/ME20 S, ME20-M, ME20-S, ME20M, ME20M/ME20S, ME20S, Melanocyte lineage specific antigen GP100, Melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocyte protein Pmel 17 precursor, Melanocytes lineage-specific antigen GP100, Melanoma associated ME20 antigen, Melanoma gp100, Melanoma-associated ME20 antigen, Melanosomal matrix protein 17, Melanosomal matrix protein17, P1, p100, p26, PMEL 17, PMEL, PMEL_HUMAN, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SI, SIL, SILV, Silver(mouse homolog) like, Silver homolog, Silver locus protein homolog, Silver, mouse, homolog of.

Product Description

Melanoma gp100 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 68 kDa
• UniProt: P40967
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: PMEL

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (Human melanoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Melanoma gp100) Polyclonal Antibody, Unconjugated (orb185162) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: U251 (Human) Cell Lysate at 30 ug, ASPC-1 (Human) Cell Lysate at 30 ug, MDA-MB-231 (Human) Cell Lysate at 30 ug, Primary: Anti-Melanoma gp100 (orb185162) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 62 kD.