E.coli-derived human AMH recombinant protein (Position: A75-E141). Human AMH shares 66.7% amino acid (aa) sequence identity with both mouse and rat AMH.
Application Notes: Western blot, 0.1-0.5µg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
WB analysis of AMH using anti-AMH antibody.Lane 1:293T cell,2:COLO320 cell.
Western blot analysis of AMH using anti-AMH antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: 293T whole Cell lysates, Lane 2: COLO320 whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMH antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AMH4 at approximately 65KD. The expected band size for AMH is at 59KD.
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