D4S234E Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: D4S234E Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB1876
• Supplier Catalog Number: orb1876
• Alternative Catalog Number: BYT-ORB1876-50,BYT-ORB1876-100,BYT-ORB1876-200
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human NEEP21 (1-100/185aa)
• Conjugation: Unconjugated
• Alternative Names: D4S234, D4S234E, NEEP21, P21, m234, Bsmrb, PEP1, NSG1_HUMAN, NSG1, Neuron-enriched endosomal protein of 21 kDa, Neuron-specific protein family member 1, NSG1_MOUSE, NSG1_RAT, Brain neuron cytoplasmic protein 1/2,

Product Description

D4S234E Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 21 kDa
• UniProt: P42857
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: NSG1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (D4S234E) Polyclonal Antibody, Unconjugated (orb1876) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (D4S234E) Polyclonal Antibody, Unconjugated (orb1876) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (D4S234E) Polyclonal Antibody, Unconjugated (orb1876) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Cerebral cortex (Mouse) Lysate at 40 ug, Primary: Anti-D4S234E (orb1876) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 21 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-D4S234E (orb1876) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 21 kD.

Sample: Hippocampus (Mouse) Lysate at 40 ug, Primary: Anti-D4S234E (orb1876) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 21 kD.

IHC-P analysis of Mouse brain using D4S234E Polyclonal Antibody at 1:400 dilution.

IHC-P analysis of rat cerebellum using D4S234E Polyclonal Antibody at 1:200 dilution.