Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application Dilute:
FC - 1:25, WB - 1:2000
Overlay histogram showing Jurkat cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG (1 µg/1x10 6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
All lanes: Anti-VE Cadherin Antibody (CDH5) (N-term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 88 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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