Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Application Dilute:
WB - 1:1000
Western blot analysis of mouse anti-Semaphorin 5A Pab in mouse brain tissue lysates (35 ug/lane). Semaphorin 5A (arrow) was detected using the purified Pab.
Anti-Semaphorin 5A (C-term) at 1:1000 dilution + U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 121 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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