Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application Dilute:
WB - 1:1000
NROB1 Antibody (N-term) western blot analysis in Hela, NCI-H292, PC-3 cell line lysates (35 ug/lane). This demonstrates the NROB1 antibody detected the NROB1 protein (arrow).
All lanes: Anti-NROB1 Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: NCI-H460 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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