Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application Dilute:
WB - 1:1000
Western blot analysis of anti-TRIM69 Antibody (Center H215) in K562 cell line lysates (35 ug/lane). TRIM69 (arrow) was detected using the purified Pab.
Anti-TRIM69 Antibody (Center H215) at 1:1000 dilution + PC-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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