Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application Dilute:
WB - 1:1000, IHC-P - 1:100-500
YY1 Antibody (L89) western blot analysis in 293, HL-60, SiHa, K562, THP-1, U266 cell line lysates (35 ug/lane). This demonstrates the YY1 antibody detected the YY1 protein (arrow).
All lanes: Anti-YY1 Antibody (N-term) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: C2C12 whole cell lysate. Lane 3: C6 whole cell lysate. Lane 4: Daudi whole cell lysate. Lane 5: U-251 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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