Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application Dilute:
WB - 1:1000
Western blot analysis of ART3 Antibody (C-term) in HL60 cell line lysates (25 ug/lane). ART3 (arrow) was detected using the purified Pab (4 ug/ml).
Anti-ART3 Antibody at 1:4000 dilution + human testis lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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