Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Target:
This HOXC10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-250 amino acids from the C-terminal region of human HOXC10.
Application Dilute:
IF: 1:10~50, WB: 1:1000
Fluorescent image of Hela cell stained with HOXC10 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXC10 primary antibody (1:25, 1 h at 37C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37C).HOXC10 immunoreactivity is localized to Nucleus significantly.
HOXC10 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35 ug/lane). This demonstrates the HOXC10 antibody detected the HOXC10 protein (arrow).
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