Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application Dilute:
WB-1:1000, FC-1:10~50, IF-1:10~50, IHC-P-1:10~50
Confocal immunofluorescent analysis of TRIM65 Antibody (Center) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
TRIM65 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the TRIM65 antibody detected the TRIM65 protein (arrow).
TRIM65 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TRIM65 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
TRIM65 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of TRIM65 Antibody (Center) (orb1937931) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
TRIM65 Antibody (Center) (orb1937931) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TRIM65 Antibody (Center) detected the TRIM65 protein (arrow).
TRIM65 Antibody (Center) (orb1937931) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TRIM65 Antibody (Center) (orb1937931) for immunohistochemistry. Clinical relevance has not been evaluated.
TRIM65 Antibody (Center) (orb1937931) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis
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