ELISA 1:5000-10000 Western blotting 1:1000-2000 ICH 1:1000-2000
Microtiter wells were coated with human PLXDC2-ECD (extracellular domain, truncated a transmembrane domain from PLXDC2) at 5 ug/mL. Anti-PLXDC2 (4G3) rabbit mAb Biotin Conjugate (Catorb1945972) was diluted at 1 ug/mL and 0.1 ug/mL. Anti-PLXDC2 (4G3) rabbit mAb Biotin Conjugate show very specific binding to human PLXDC2-ECD antigen.
A sandwich ELISA was performed using anti-PLXDC2 specific rabbit mAb: 3B8 (CatXXXX) as a capture antibody and 4G3-biotin (Catorb1945972) as a detection antibody. Human PLXDC2-ECD was serially diluted 1:1 starting at 5 ug/mL. Anti-PLXDC2 rabbit mAb 3B8 and 4G3-biotin detected hPLXDC2-ECD antigen at very high sensitivity as low as 100 pg/mL.
Western blot analysis of extracts from HEK293 cells, untransfected or transfected with human PLXDC2 gene, with anti-PLXDC2 (4G3) rabbit mAb Biotin Conjugate at 0.5 µg/mL Cat. orb1945972
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