Used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP) in immunoassay. Can be paired with other NP-specific clones for detection in sandwich ELISA format.
Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) at 1 ug/mL. Biotin-conjugated rabbit monoclonal antibody 85C10 (Cat orb1945982) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to COVID-19 NP antigen.
A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 75G5a (Cat orb1945992) as a capture antibody and 85C10-biotin (Cat orb1945982) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 20 ng/mL. Rabbit monoclonal antibodies 75G5a and 85C10-biotin detected COVID-19 NP antigen at very high sensitivity as low as 19.5 pg/mL (1.9 pg).
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