Used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP) in immunoassay. Can be paired with other NP-specific clones for detection in sandwich ELISA format.
Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody 85C1 (Cat orb1945986) was serially diluted 1:2 starting at 1 ug/mL. Rabbit monoclonal antibody 85C1 show very strong and specific binding to both COVID-19 NP antigen and to SARS-CoV NP antigen.
A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 85C1 (Cat orb1945986) as a capture antibody and 75G5a-biotin (Cat orb1945991) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 85C1 and 75G5a-biotin detected COVID-19 NP antigen at very high sensitivity as low as 25 pg/mL (2.5 pg).
Western blot analysis of 50 ng of SARS-CoV2 and SARS-CoV nucleocapsid protein probed with 1 ug/mL CoV2NP rabbit monoclonal antibody (85C1), CoV2NP-84C1. Cat. orb1945986.
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