SARS-CoV-2 NP (75G5a) rabbit mAb Biotin Conjugate Antibody, Clone: [75G5a], Rabbit, Monoclonal

Product Overview

• Article Name: SARS-CoV-2 NP (75G5a) rabbit mAb Biotin Conjugate Antibody, Clone: [75G5a], Rabbit, Monoclonal
• Biozol Catalog Number: BYT-ORB1945991
• Supplier Catalog Number: orb1945991
• Alternative Catalog Number: BYT-ORB1945991-20,BYT-ORB1945991-200
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Other
• Immunogen: SARS-CoV-2 nucleoprotein (NP) specific peptide
• Conjugation: Biotin
• Alternative Names: 2019-nCoV nucleocapsid protein, Severe acute respiratory syndrome coronavirus 2 nucleoprotein, sars-cov-2

Product Description

SARS-CoV-2 NP (75Ga) rabbit mAb Biotin Conjugate for immunoassay. Biorbyt creates robustly validated, high quality recombinant mAbs.

Product Properties

• Clonality: Monoclonal
• Concentration: 0.5 mg/mL
• Clone Designation: [75G5a]
• UniProt: P0DTC9
• Buffer: 1X PBS, 0.02% NaN3
• Form: Liquid

Application Notes

• Application Dilute: Used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP) in immunoassay. Can be paired with other NP-specific clones for detection in sandwich ELISA format.

Images

Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) at 1 ug/mL. Biotin-conjugated rabbit monoclonal antibody 75G5a (Cat orb1945991) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to COVID-19 NP antigen.

Western blot analysis of SARS-CoV-2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP nucleocapsid rabbit monoclonal antibody (75G5), CoV2NP-75G5a), Cat orb1945992.

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 84C4a (Cat orb1945990) as a capture antibody and 75G5a-biotin (Cat orb1945991) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 84C4a and 75G5a-biotin detected COVID-19 NP antigen at very high sensitivity as low as 49 pg/mL (4.9 pg).

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 85C1 (Cat orb1945986) as a capture antibody and 75G5a-biotin (Cat orb1945991) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 85C1 and 75G5a-biotin detected COVID-19 NP antigen at very high sensitivity as low as 25 pg/mL (2.5 pg).