Human 3-Hydroxyanthranilate 3, 4-Dioxygenase (HAAO) ELISA Kit

Catalog Number: BYT-ORB1948829
Article Name: Human 3-Hydroxyanthranilate 3, 4-Dioxygenase (HAAO) ELISA Kit
Biozol Catalog Number: BYT-ORB1948829
Supplier Catalog Number: orb1948829
Alternative Catalog Number: BYT-ORB1948829-48,BYT-ORB1948829-96
Manufacturer: Biorbyt
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: 3HAO
Human 3-Hydroxyanthranilate 3, 4-Dioxygenase (HAAO) ELISA Kit
Range: 31.25-2000pg/mL
Sensitivity: 18.75 pg/mL
Samples: serum, plasma, Tissue homogenate and Other biological samples
Target: HAAO
Application Notes: Application Notes: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HAAO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HAAO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HAAO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Human HAAO. You can calculate the concentration of Human HAAO in the samples by comparing the OD of the samples to the standard curve
orb1948829
orb1948829