Application Notes: Western blot, 0.1-0.5µg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, Chicken Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Mouse, Rat Immunocytochemistry , 0.5-1µg/ml, Human Immunofluorescence, 2µg/ml, Human, Mouse, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
IF analysis of CD3E and CD20 using anti-CD3E antibody and anti-CD20 antibody. CD3E and CD20 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/mL rabbit anti-CD3E antibody and mouse anti-CD20 antibody overnight at 4C. DyLight550 Conjugated Goat Anti-Rabbit IgG, DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of CD3E and CD68 using anti-CD3E antibody and anti-CD68 antibody. CD3E and CD68 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/mL rabbit anti-CD3E Antibody and mouse anti CD68 Antibody overnight at 4C. DyLight488 Conjugated Goat Anti-Mouse IgG and Biotin conjugated goat anti-rabbit IgG were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The tissue section was developed using Cy3 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody. CD3 Epsilon was detected in frozen section of mouse spleen tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CD3 Epsilon Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody. CD3 Epsilon was detected in frozen section of rat spleen tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CD3 Epsilon Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody. CD3 Epsilon was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CD3 Epsilon Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody. CD3 Epsilon was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CD3 Epsilon Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody. CD3 Epsilon was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissu
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