Phospho-STMN1 (Ser63) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-STMN1 (Ser63) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB1966
• Supplier Catalog Number: orb1966
• Alternative Catalog Number: BYT-ORB1966-50,BYT-ORB1966-100,BYT-ORB1966-200
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Stathmin 1 around the phosphorylation site of Ser63 (41-140/149aa)
• Conjugation: Unconjugated
• Alternative Names: STMN1 (p-S63), p-STMN1, phospho-STMN1, C1orf215, LAP18, Lag, OP18, PP17, PP19, PR22, SMN, 19k, P18, P19, Pig, Pp18, prosolin, STMN1_HUMAN, STMN1, Leukemia-associated phosphoprotein p18, Metablastin, Oncoprotein 18 (Op18), Phosphoprotein p19 (pp19), Protein Pr22, STMN1_MOUSE, Leukemia-associated gene protein, STMN1_RAT, Pr22 protein,

Product Description

Phospho-STMN1 (Ser63) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 16 kDa
• UniProt: P16949
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: STMN1

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
• Application Notes: Modification: Phosphorylated

Images

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (STMN1) Polyclonal Antibody, Unconjugated (orb1966) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (STMN1) Polyclonal Antibody, Unconjugated (orb1966) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-STMN1 (Ser63)) Polyclonal Antibody, Unconjugated (orb1966) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (STMN1) Polyclonal Antibody, Unconjugated (orb1966) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-STMN1 (Ser63)) Polyclonal Antibody, Unconjugated (orb1966) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Positive control: (mo) Thymocyte (2% Paraformaldehyde-fixed), Isotype Control Antibody: Rabbit IgG dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Secondary Antibody: Goat anti-rabbit IgG-FITC dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody: rabbit Anti-phospho-Stathmin 1 (Ser63) (orb1966) dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA.

Flow cytometric analysis of (mo) Thymocyte Cell using pStathmin 1 antibody.