The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VAP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VAP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VAP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human VAP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Concentration:
2000 pg/mL
Range:
31.25-2000 pg/mL
Sensitivity:
12.1 pg/mL
Samples:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Application Notes: standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VAP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VAP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VAP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human VAP-1 in the samples is then determined by comparing the OD of the samples to the standard curve
* VAT and and shipping costs not included. Errors and price changes excepted
