UNG Heat Labile

Product Overview

• Article Name: UNG Heat Labile
• Biozol Catalog Number: BYT-ORB1994997
• Supplier Catalog Number: orb1994997
• Alternative Catalog Number: BYT-ORB1994997-100, BYT-ORB1994997-500, BYT-ORB1994997-5000
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Alternative Names: UDG, b2580, JW2564, EC 3.2.2.27, DGU, UNG15, HIGM5, Uracil-DNA Glycosylase 1, EC 3.2.2, HIGM4, UNG2.

Product Description

Recombinant Psychrophilic Marine Bacterium Uracil DNA Glycosylase, Heat Labile

Product Properties

• Buffer: UNG protein solution (1U/ul) 20mM Tris-HCl (25°C, pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% NP-40, 0.5% Tween-20 and 50% glycerol.
• Purity: Greater than 97.0% as determined by SDS-PAGE.
• Form: Sterile Filtered clear solution.

Application Notes

• Application Notes: Application Notes: Applications: Treatment of 0.1µg of uracil containing DNA with 1U UDG for 10 min. at 37°C renders the DNA incapable of being copied by DNA polymerase. The enzyme can be 95% heat killed by incubation at 95°C for 10 minutes. Since UDG remains partially active following heat treatment at 95°C, it is recommended that uracil glycosylase inhibitor be added to prevent degradation of product DNA. Alternatively, reaction products can be immediately extracted with phenol/chloroform. Unit Definition: 1 unit is defined as the amount of enzyme that releases 1 nmol of uracils from the DNA strand (containing dU) within 1 hour at 37C in the reaction system containing 70mM TrisHCl, pH-7.5, 10mM NaCl, 1mM EDTA and 0.1mg/ml BSA reaction liquid