Application Notes: Applications: Gibson Assembly. Unit Definition: 1 unit of T5 Exonuclease is defined as the amount of enzyme required to cause the change of 0.00032 A260nm/min at 37 C in 1xReaction Buffer: 20mM Tris-acetate (pH 7.9 25C), 50mM Potassium Acetate, 10mM Magnesium Acetate and 1mM DTT
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