Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Sequence:
CKIFKHYKDSLQ
Target:
Mcp10 / Mcp9
Application Dilute:
ELISA: 1:2000, WB: 0.02 µg/ml
Application Notes:
Application Notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Preliminary experiments gave an approx 49kDa band in Human Skeletal Muscle lysates after 0.02 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 26.9kDa according to NP_001019885.1 and NP_034912.3. The 49kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
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