Goat Rat IgG2b (subclass specific), conjugated with Biotin Antibody, Polyclonal
Biozol Catalog Number:
BYT-ORB21351
Supplier Catalog Number:
orb21351
Alternative Catalog Number:
BYT-ORB21351-1
Manufacturer:
Biorbyt
Host:
Goat
Category:
Antikörper
Species Reactivity:
Rat
Conjugation:
Biotin
Biotin-conjugated IgG fraction of polyclonal goat antiserum to rat IgG2b, subclass specific. In immunocytochemical and immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgG2b antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2b isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG2b in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the users choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000 depending on the method used.
Clonality:
Polyclonal
Form:
Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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