Goat Rat IgG1 IgG2a IgG2b IgG2c (Fc specific), conjugated with Biotin Antibody, Polyclonal
Biozol Catalog Number:
BYT-ORB21694
Supplier Catalog Number:
orb21694
Alternative Catalog Number:
BYT-ORB21694-1
Manufacturer:
Biorbyt
Host:
Goat
Category:
Antikörper
Species Reactivity:
Rat
Conjugation:
Biotin
Biotin-conjugated IgG fraction of polyclonal goat antiserum to rat IgG, Fc specific. In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of rat origin known to be of the IgG isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the users choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:15,000.
Clonality:
Polyclonal
Form:
Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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