Donkey Goat IgG (heavy and light chains), conjugated with Biotin Antibody, Polyclonal
Biozol Catalog Number:
BYT-ORB22021
Supplier Catalog Number:
orb22021
Alternative Catalog Number:
BYT-ORB22021-1
Manufacturer:
Biorbyt
Host:
Donkey
Category:
Antikörper
Species Reactivity:
Goat
Conjugation:
Biotin
Biotin-conjugated IgG fraction of polyclonal donkey antiserum to goat IgG, heavy and light chains. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immuno- fluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of goat origin in the middle layer of the indirect test procedure. As an extra step an avidin or streptavidin of the users choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions fir histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable no-precipitating antibody-binding assays between 1: 1,000 and 1:10,000.
Clonality:
Polyclonal
Form:
Biotin-coupled purified hyperimmune donkey IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
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