MX2 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB221565
Article Name: MX2 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB221565
Supplier Catalog Number: orb221565
Alternative Catalog Number: BYT-ORB221565-100,BYT-ORB221565-200,BYT-ORB221565-50
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P
Species Reactivity: Human
Immunogen: KLH conjugated synthetic peptide derived from human MX2 (621-715/715aa)
Conjugation: Unconjugated
Alternative Names: Interferon induced GTP binding protein Mx2, Interferon regulated resistance GTP binding protein MXB, Interferon-induced GTP-binding protein Mx2, Interferon-regulated resistance GTP-binding protein MxB, MX 2, MX dynamin like GTPase 2, Mx2, MX2_HUMAN, MXB, Myxovirus(influenza virus) resistance 2, Myxovirus resistance 2, Myxovirus resistance protein 2, p78 related protein, p78-related protein, Second interferon induced protein p78.
MX2 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 79 kDa
UniProt: P20592
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: MX2
Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of human lung carcinoma tissue using MX2 antibody.
Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (MX2) polyclonal Antibody, Unconjugated (orb221565) 1:25, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-MX2 Polyclonal Antibody, Unconjugated (orb221565) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.