Nesprin 3 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Nesprin 3 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB221708
• Supplier Catalog Number: orb221708
• Alternative Catalog Number: BYT-ORB221708-100,BYT-ORB221708-200,BYT-ORB221708-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Nesprin 3 (201-300/975aa)
• Conjugation: Unconjugated
• Alternative Names: C14orf49, Chromosome 14 open reading frame 49, FLJ25605, MGC75267, Nesp3, Nesprin3, Nesprin-3, NET53, Nuclear Envelope Spectrin Repeat Protein 3, Spectrin Repeat Containing, Nuclear Envelope Family Member 3, SYNE3, SYNE3_HUMAN.

Product Description

Nesprin 3 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 112 kDa
• UniProt: Q6ZMZ3
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: SYNE3

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (orb221708) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (orb221708) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (orb221708) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (orb221708) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (orb221708) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (orb221708) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Hepg2 (Human) Cell Lysate at 30 ug, Primary: Anti-Nesprin 3 (orb221708) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 112 kD, Observed band size: 112 kD.

IHC-P analysis of Rat brain using Nesprin 3 Polyclonal Antibody at 1:400 dilution.

IHC-P analysis of Mouse brain using Nesprin 3 Polyclonal Antibody at 1:400 dilution.