The C-terminus fragment of recombinant human Estrogen Receptor beta protein was used as the immunogen for this antibody.
Conjugation:
Unconjugated
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including alpha and beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER alpha and beta are differentially activated by various ligands. Ligand interaction triggers a cascade of events, including dissociation from heat shock proteins, recepter dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER alpha and beta may be regulated by distinct mechanisms even though they share many functional characteristics.
Clonality:
Monoclonal
Clone Designation:
[ERb455]
Formula:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Application Dilute:
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10 6 cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml
Application Notes:
Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Estrogen Receptor beta antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
IHC testing of human breast cancer stained with Estrogen Receptor beta antibody (ERb455).
IHC testing of human ovarian cancer stained with Estrogen Receptor beta antibody (ERb455).
FACS testing of human MCF-7 cells: Black = cells alone, Green = isotype control, Red = Estrogen Receptor beta antibody PE conjugate
FACS testing of human BT474 cells: Black = cells alone, Gray = isotype control, Green = Estrogen Receptor beta antibody AF488 conjugate
IHC testing of human gastric carcinoma stained with Estrogen Receptor beta antibody (ERb455).
IHC testing of human bladder carcinoma stained with Estrogen Receptor beta antibody (ERb455).
Western blot testing of human MCF7 cell lysate with Estrogen Receptor beta antibody (clone ERb455). Expected molecular weight: 53-59 kDa.
SDS-PAGE analysis of purified, BSA-free Estrogen Receptor beta antibody (clone ERb455) as confirmation of integrity and purity.
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