Human PCSK9 Protein, His Tag, Unconjugated

Product Overview

• Article Name: Human PCSK9 Protein, His Tag, Unconjugated
• Biozol Catalog Number: BYT-ORB257745
• Supplier Catalog Number: orb257745
• Alternative Catalog Number: BYT-ORB257745-50,BYT-ORB257745-1
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Unconjugated

Product Description

Human PCSK9 (D374Y), His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession Q8NBP7-1 (D374Y)).

Product Properties

• Molecular Weight: 13.8 kDa and 58.2 kDa
• NCBI: 374
• UniProt: Q8NBP7
• Buffer: PBS, pH 7.4
• Source: Human
• Purity: 90%
• Form: Powder
• Sequence: AAI66619.1 (D374Y)
• Target: PCSK9

Application Notes

• Application Notes: Application Notes: This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non-covalent interactions and with a calculated MW of 13.8 kDa and 58.2 kDa respectively. The protein migrates as 15 kDa and 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. The D374Y mutation results in higher affinity of PCSK9 for LDLR

Images

Line graph illustrates about the sensitivity range of Human PCSK9 protein using different concentrations

SDS-PAGE analysis of Human PCSK9 protein