Human TNF-alpha Protein, Unconjugated

Product Overview

• Article Name: Human TNF-alpha Protein, Unconjugated
• Biozol Catalog Number: BYT-ORB257883
• Supplier Catalog Number: orb257883
• Alternative Catalog Number: BYT-ORB257883-1,BYT-ORB257883-100,BYT-ORB257883-20
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Unconjugated

Product Description

Human TNF-alpha, premium grade is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession P01375-1).

Product Properties

• Molecular Weight: 17.4 kDa
• NCBI: 000585
• UniProt: P01375
• Buffer: PBS, pH 7.4
• Source: Human
• Purity: 95%
• Form: Powder
• Sequence: NP_000585.2
• Target: TNF-alpha

Application Notes

• Application Notes: Application Notes: This protein carries no tag. The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation

Images

Line graph illustrates about the sensitivity range of Human TNF-alpha protein using different concentrations

SDS-PAGE analysis of Human TNF-alpha protein

Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade (Cat. No. orb257883) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).