Human VEGF165 Protein, His Tag, low endotoxin (MALS verified), Unconjugated

Product Overview

• Article Name: Human VEGF165 Protein, His Tag, low endotoxin (MALS verified), Unconjugated
• Biozol Catalog Number: BYT-ORB257924
• Supplier Catalog Number: orb257924
• Alternative Catalog Number: BYT-ORB257924-1,BYT-ORB257924-50
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Unconjugated

Product Description

Human VEGF165, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession P15692-4).

Product Properties

• Molecular Weight: 21.1 kDa
• NCBI: 001165097
• Pubmed: 28468826
• UniProt: P15692
• Buffer: PBS, pH 7.4
• Source: Human
• Purity: 90%
• Form: Powder
• Sequence: NP_001165097.1
• Target: VEGF165

Application Notes

• Application Notes: Application Notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 21.1 kDa. The protein migrates as 27-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation

Images

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

SDS-PAGE analysis of Human VEGF protein

Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165 Protein, His Tag (Cat. No. orb257924) with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).