Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Source:
Rat
Purity:
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG, anti-Rat IgG F(ab)2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin.
Form:
Lyophilized
Application Notes:
Biological Origin: Rat. Application Notes: Rat IgG F(ab)2 Fragment has been tested in SDS-Page and are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 1.0 mL
SDS-PAGE of Rat IgG F(c) Fragment Rhodamine Conjugated. Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule (p/n orb2652744). Lane 2: Reduced Rat IgG F(c) Fragment Rhodamine Conjugated. Lane 3: Reduced Rat IgG Fab Fragment (p/n orb346340). Lane 4: Reduced Rat IgM Whole Molecule (p/n orb346341). Load: 1 µg of IgG, F(c), Fab, 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa, F(c) at 25 kDa, Fab at 25 kDa, IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.
SDS-PAGE Results of Rat F(ab)2 fragment (p/n orb2652742). Lane 1: Rat F(ab)2 Reduced 10 µg. Lane 2: Rat F(ab)2 Reduced 5 µg. Lane 3: Rat F(ab)2 Reduced 1 µg. Lane 4: Opal Prestained Molecular Weight Marker. Lane 5: Rat F(ab)2 Non-Reduced 10 µg. Lane 6: Rat F(ab)2 Non-Reduced 5 µg. Lane 7: Rat F(ab)2 Non-Reduced 1 µg. 4-20% Gel, Coomassie stained.
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