Rat IgG whole molecule, Unconjugated Preis auf Anfrage

Product Overview

• Article Name: Rat IgG whole molecule, Unconjugated Preis auf Anfrage
• Biozol Catalog Number: BYT-ORB2652743
• Supplier Catalog Number: orb2652743
• Alternative Catalog Number: BYT-ORB2652743-1
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Application: SDS-PAGE, WB
• Conjugation: Unconjugated
• Alternative Names: Rat Immunoglobulin G, Rat IgG whole molecule, Rat IgG purified protein

Product Description

Rat IgG whole molecule

Product Properties

• Concentration: 40 mg/mL
• Buffer: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Source: Rat
• Purity: Rat IgG was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat Serum.
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: ELISA: User Optimized, FC: User Optimized, WB: User Optimized
• Application Notes: Biological Origin: Rat. Application Notes: Rat IgG while molecule has been tested by SDS-Page and western blot is suitable for use as antigen or ligand in immunochemical reactions, as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified immunoglobulins

Images

SDS PAGE of Rat IgG Whole Molecule. Lane 1: Non-Reduced Rat IgG Whole Molecule. Lane 2: 5 µl Opal Prestained Marker. Lane 3: Reduced Rat IgG Whole Molecule. Load: 1 µg per lane. Predicted/Observed size: Non-Reduced at 160 kDa/Observed at 245 kDa, Reduced at 55, 25 kDa. Non-reduced IgG migrates slightly higher.

SDS-PAGE of Rat IgG F(c) Fragment Rhodamine Conjugated. Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule (p/n orb2652744). Lane 2: Reduced Rat IgG F(c) Fragment Rhodamine Conjugated. Lane 3: Reduced Rat IgG Fab Fragment (p/n orb346340). Lane 4: Reduced Rat IgM Whole Molecule (p/n orb346341). Load: 1 µg of IgG, F(c), Fab, 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa, F(c) at 25 kDa, Fab at 25 kDa, IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.