Rabbit IgG whole molecule, Unconjugated Preis auf Anfrage

Product Overview

• Article Name: Rabbit IgG whole molecule, Unconjugated Preis auf Anfrage
• Biozol Catalog Number: BYT-ORB2652746
• Supplier Catalog Number: orb2652746
• Alternative Catalog Number: BYT-ORB2652746-1
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Application: SDS-PAGE
• Conjugation: Unconjugated
• Alternative Names: Rabbit immunoglobulin G

Product Description

Rabbit IgG whole molecule

Product Properties

• Concentration: 10 mg/mL
• Buffer: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Source: Rabbit
• Purity: Rabbit IgG whole molecular was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Rabbit IgG whole molecular was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Rabbit Serum.
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
• Application Notes: Biological Origin: Rabbit. Application Notes: Rabbit IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments

Images

SDS-PAGE of Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane 2: Reduced Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n orb346310). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n orb346313). Load: 1 µg for F(ab) and F(c), 1.2 µg for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

SDS-PAGE of Rabbit IgG Whole Molecule. Lane 1: Non-reduced Rabbit IgG Whole Molecule. Lane 2: 5 µl OPAL Pre-stained Marker. Lane 3: Reduced Rabbit IgG Whole Molecule. Load: 1 µg per lane. Predicted/Observed size: Non-reduced at 150-170 kDa, Reduced at 55, 25 kDa.