Mouse IgG whole molecule, Unconjugated Preis auf Anfrage

Product Overview

• Article Name: Mouse IgG whole molecule, Unconjugated Preis auf Anfrage
• Biozol Catalog Number: BYT-ORB2652748
• Supplier Catalog Number: orb2652748
• Alternative Catalog Number: BYT-ORB2652748-1
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Application: ELISA, SDS-PAGE
• Conjugation: Unconjugated
• Alternative Names: MOUSE IgG whole molecule, Mouse Immunoglobulin G

Product Description

Mouse IgG whole molecule

Product Properties

• Concentration: 20 mg/mL
• Buffer: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Source: Mouse
• Purity: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum.
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: ELISA: User Optimized, FC: User Optimized, IHC: User Optimized, IP: User Optimized, WB: User Optimized
• Application Notes: Biological Origin: Mouse. Application Notes: Mouse IgG whole molecule has been tested by ELISA and SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments

Images

SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). MW: 5 µl Opal Prestained Marker. Lane 1: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 2: Reduced Mouse F(c) Fragment (p/n orb346280). Lane 3: Reduced Mouse F(ab) Fragment (p/n orb346282). Lane 4: Mouse IgM Kappa Myeloma Protein. Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

SDS-Page of Mouse IgG whole molecule. Lane 1: Mouse IgG reduced. Lane 2: Molecular Weight Marker. Lane 3: Mouse IgG non-reduced. Load: 1 µg per lane. Predicted/Observed size (non-reduced): 160 kDa, 160 kDa. Predicted/Observed size (reduced): 55 and 28 kDa, 55 and 28 kDa.