Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Source:
Human
Purity:
Human IgA whole molecule was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Human IgA whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgA (a chain specific). No reaction was observed against anti-Human IgG F(c). Some light chain cross reactivity will occur with anti-Human IgG.
Form:
Liquid (sterile filtered)
Application Dilute:
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Application Notes:
Biological Origin: Human. Application Notes: Human IgA whole molecule has been tested in SDS-Page and western blot and can be utilized as a control or standard reagent in SDS, Western Blotting and ELISA experiments
Identification of b12A2 antibody secreted in the milk of transgenic mice using western blotLanes 1-8 are milk samples collected from individual mice, lane 9 is the molecular weight marker, lane 10 is milk from a negative control mouse, lane 11 is a known concentration of standard human IgA (p/n orb2652753) at 80 ng/lane, lane 12 is negative control mouse milk spiked with standard human IgA. 80 ng of IgA standard is equivalent to 0.8 mg/ml in milk when loaded at 0.1 µl of milk per lane. Reactive bands were detected using biotinylated goat anti-human IgA alpha chain (p/n orb347244) followed by HRP conjugated streptavidin-IR800CW.
SDS-PAGE of Human IgA Whole Molecule. Lane 1: Human IgA Whole Molecule, Reduced [5.0 µg]. Lane 2: Human IgA Whole Molecule, Reduced [1.0 µg]. Lane 3: Opal Prestained Molecular Weight Marker. Lane 4: Human IgA Whole Molecule, Non-Reduced [1.0 µg]. Lane 5: Human IgA Whole Molecule, Non-Reduced [5.0 µg]. 4-20% Gel, Coomassie Blue Stained. Expected MW: ~160 kDa Non-Reduced, ~55kDa, and 23kDa Reduced. Additional bands: monomer, dimer and trimer.
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