Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Source:
Goat
Purity:
Goat IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Goat IgG whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat IgG and anti-Goat Serum.
Form:
Lyophilized
Application Dilute:
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Application Notes:
Biological Origin: Goat. Application Notes: Goat IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 2.5 mL
SDS-PAGE of Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane M: 5 µl Opal Prestained Marker. Lane 1: Reduced Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane 2: Reduced Goat IgG F(c) Fragment (p/n orb2652765). Lane 3: Reduced Goat IgG F(ab) Fragment (p/n orb2652763). Lane 4: Reduced Goat IgM Whole Molecule (p/n orb346110). Load: 1 µg for IgG, F(c) and F(ab), 3 µg for IgM. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
SDS-PAGE of Goat IgG Whole Molecule. Lane 1: Reduced Goat IgG Whole Molecule. Lane 2: 3 µl OPAL Pre-stained Marker. Lane 3: Non-reduced Goat IgG Whole Molecule. Load: 1 µg per lane. Predicted/Observed size: Non-reduced at 160 kDa/observed at 180-200 kDa, Reduced at 55, 25 kDa. Non-reduced migrates at slightly higher molecular weight.
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