Chicken IgG F(ab)2, Unconjugated Preis auf Anfrage

Product Overview

• Article Name: Chicken IgG F(ab)2, Unconjugated Preis auf Anfrage
• Biozol Catalog Number: BYT-ORB2652773
• Supplier Catalog Number: orb2652773
• Alternative Catalog Number: BYT-ORB2652773-10
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Application: SDS-PAGE
• Conjugation: Unconjugated
• Alternative Names: Chicken IgG F(ab)2 fragment, Chicken IgG Fab2 fragment, Chicken IgY F(ab)2 fragment, Chicken IgY Fab2 fragment

Product Description

Chicken IgG F(ab)2

Product Properties

• Concentration: 10.0 mg/mL
• Buffer: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Source: Chicken
• Purity: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken IgG, anti-Chicken IgG F(ab)2 and anti-Chicken Serum. No reaction was observed against anti-Chicken IgG F(c) or anti-Pepsin.
• Form: Lyophilized

Application Notes

• Application Notes: Biological Origin: Chicken. Application Notes: Chicken IgG F(ab)2 fragment has been tested by SDS-PAGE and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 1.0 mL

Images

SDS-PAGE of Chicken IgG/IgY Whole Molecule Rhodamine Conjugated (p/n orb346044). Lane M: 5 µl Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule Rhodamine Conjugated (p/n orb346044). Lane 2: Reduced Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Reduced Chicken IgG Fab Fragment (p/n orb2652771). Lane 4: Reduced Chicken IgM Whole Molecule (p/n orb346054). Load: 1 µg per lane. Predicted/Observed size: IgG at 72 and 25 kDa, F(c) at 25 kDa, Fab at 25 kDa, IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.

SDS-PAGE Results of Chicken IgG F(ab)2. Lane 1: Chicken IgG F(ab)2 fragment - Reduced [5 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Chicken IgG F(ab)2 fragment - Non-Reduced [5 µg]. 4-20% Gel. Coomassie stained.