HDAC2 Antibody (C-term), Clone: [RB5718], Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: HDAC2 Antibody (C-term), Clone: [RB5718], Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB28974
• Supplier Catalog Number: orb28974
• Alternative Catalog Number: BYT-ORB28974-80
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Synthetic Peptide
• Conjugation: Unconjugated
• Alternative Names: Anti-Histone deacetylase 2 antibody, anti-HD2 antibody

Product Description

Purified rabbit polyclonal antibody.

Product Properties

• Clonality: Polyclonal
• Clone Designation: [RB5718]
• Molecular Weight: 55364
• NCBI: 001518
• UniProt: Q92769
• Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Target: HDAC2

Application Notes

• Application Dilute: WB: 1:1000, IF/ICC: 1:10-50, IHC-P: 1:10-50

Images

WB analysis of 293 cell line lysate using HDAC2 antibody [Out of Stock] (dilution at 1:1000)

orb28974

Confocal immunofluorescent analysis of HDAC2 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Fluorescent confocal image of Hela cell stained with HDAC2 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC2 primary antibody (1:25, 1 h at 37C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). hHDAC2 immunoreactivity is localized to Nucleus significantly.

Western blot analysis of HDAC2 (arrow) using rabbit polyclonal HDAC2 Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HDAC2 gene (Lane 2).

Western blot analysis of anti-HDAC2 Antibody (C-term) in 293 cell line lysates (35 ug/lane). HDAC2 (arrow) was detected using the purified Pab.

Western blot analysis of anti-HDAC2 Antibody (C-term) in mouse spleen tissue lysates (35 ug/lane). HDAC2 (arrow) was detected using the purified Pab.

HDAC2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of HDAC2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.