This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human respiratory syncytial virus A (HRSV) Fusion glycoprotein F0 has been pre-coated onto a microplate. Standards or samples are premixed with HRP-labeled antibody and then pipetted into the wells. Nirsevimab in the sample competitively binds to the pre-coated protein with HRP-labeled Nirsevimab. After washing away any unbound substances, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Nirsevimab bound in the initial step. The color development is stopped and the intensity of the color is measured.
