Human A-GHRL(AcylatedGhrelin) Microsample ELISA Kit
Biozol Catalog Number:
BYT-ORB2997297
Supplier Catalog Number:
orb2997297
Alternative Catalog Number:
BYT-ORB2997297-48,BYT-ORB2997297-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
A-GHRL
Human A-GHRL(AcylatedGhrelin) Microsample ELISA Kit
Range:
15.63-1000 pg/mL
Sensitivity:
9.24 pg/mL
Samples:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Application Notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human A-GHRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human A-GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human A-GHRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human A-GHRL in the samples is then determined by comparing the OD of the samples to the standard curve
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